ELISA

  • Auteur: J. R. Crowther
  • ISBN: 1592595294
  • Date de sortie: 1995
  • Collection: Springer Science & Business Media
  • Total Download: 9439
  • Total Read: 8656

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ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate.

ELISA Mation

  • Auteur: Jarrett N. Schmit
  • ISBN: 9780849307539
  • Date de sortie: 1994-06-14
  • Collection: CRC Press
  • Total Download: 6632
  • Total Read: 8360

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ELISA-Mation is a unique, color-animated, easy-to-operate introductory tutorial for the ELISA test widely used in medical and research laboratories. The manual uses both text and animated sequences to clearly describe the steps and reactions occurring in an ELISA test. ELISA-Mation also provides many educational uses including a review for professionals, an autotutorial for students, an adjunct to formal lectures, an in-class demonstration, and a training tool for laboratory personnel.

ELISA

  • Auteur: Robert Hnasko
  • ISBN: 9781493927418
  • Date de sortie: 2015-07-10
  • Collection: Humana Press
  • Total Download: 3193
  • Total Read: 3016

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This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage production. An introductory chapter orients the reader on the basic structure and function of immunoglobulins and their fragments while subsequent chapters outline the methodology to generate monoclonal antibodies using hybridoma technology and the general methods used to purify antibodies. Multiple chapters demonstrate how to creatively use the properties of the antibody to identify, localize and quantify target analytes to answer questions and resolve problems. The reader will learn how to use a variety of immunoassay strategies, reporters and detection systems that will undoubtedly facilitate their efforts to gain answers to their own questions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, ELISA: Methods and Protocols seeks to provide both professionals and novices with the technical information necessary for the reader to successfully use the immunoassay as part of the discovery process.

ELISA and Other Solid Phase Immunoassays

  • Auteur: D. M. Kemeny
  • ISBN: 9780471909828
  • Date de sortie: 1988
  • Collection: John Wiley & Sons
  • Total Download: 3944
  • Total Read: 4876

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This is a quick-reference manual on practical aspects of immunoassay. Providing a theoretical and practical basis for modern applications of solid-phase immunoassays, this text brings together experts who have used ELISA and other assays in a variety of fields. Contributors offer step-by-step guidance on how to use the various techniques involved in immunoassay. These techniques are extemely useful to laboratory-based researchers and technicians working on the detection of allergy, the AIDS virus, autoimmunity, etc. Chapters analyze the solid-phase supports used, the amplification systems, and the quantitation and affinity of antibodies and discuss the applications of assays to biology, immunology, and microbiology.

The ELISA Guidebook

  • Auteur: John R. Crowther
  • ISBN: 1592590497
  • Date de sortie: 2000-01-01
  • Collection: Springer Science & Business Media
  • Total Download: 1402
  • Total Read: 9587

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John R. Crowther provides today's bestselling practical guide to the understanding and application of ELISA. This important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, and statistical requirements for data examination. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology.

ELISA in the clinical microbiology laboratory

  • Auteur: T. G. Wreghitt
  • ISBN:
  • Date de sortie: 1990
  • Collection:
  • Total Download: 5476
  • Total Read: 7121

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The Night of Elisa A Gothic Novel

  • Auteur: Illustrator & Storyteller Isis Sousa
  • ISBN: 1365111512
  • Date de sortie: 2016-05-13
  • Collection: Lulu Press, Inc
  • Total Download: 8911
  • Total Read: 5681

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.:The Night of Elisa - A Gothic Novel - Illustrated:. Sometimes, life and love can follow the most obscure paths, just as they did for Elisa. Her life becomes a dark, cold, lonely cage the day the devil takes her ashis wife. He robs her of almost everything she holds dear: her health,her wealth and what is left of her family. Trapped between thenuances of life and beyond-life, Elisa finds herself struggling for abetter tomorrow. With her health deteriorating, how will she summon thecourage and strength to stand her ground? And how far will she go in the pursuit of a dream? Embark with Elisa on this puzzling Gothicadventure set in the late Victorian era, between the world of the Living and the picturesque, melancholic Duskland.
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